pCTX-myc-HIPK2 WT
(Plasmid
#32996)
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Depositing Lab
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Depositing OrganizationIcahn School of Medicine at Mount Sinai
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 32996-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 32996-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCTX-myc
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Backbone manufacturerSergei Sokol
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHIPK2
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Alt nameIMAGE clone ID: 6643438
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SpeciesX. laevis (frog)
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Entrez Genehipk2
- Promoter SCMV
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Tag
/ Fusion Protein
- 6XMyc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 32996-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 32996-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCTX-myc-HIPK2 WT was a gift from Sergei Sokol (Addgene plasmid # 32996 ; http://n2t.net/addgene:32996 ; RRID:Addgene_32996) -
For your References section:
Regulation of TCF3 by Wnt-dependent phosphorylation during vertebrate axis specification. Hikasa H, Ezan J, Itoh K, Li X, Klymkowsky MW, Sokol SY. Dev Cell. 2010 Oct 19;19(4):521-32. 10.1016/j.devcel.2010.09.005 PubMed 20951344
Map uploaded by the depositor.
