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pT7-M1-S1-S2-S4T1L
(Plasmid #33306)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 33306 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 33306-D50 50 μg of DNA in Tris buffer $495
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DNA 33306-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    p3E5EGFP
  • Backbone manufacturer
    made by Yoshihiro Kawaoka
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    S2
  • Species
    Orthoreovirus T1L
  • Entrez Gene
    MoV1sS2gp1 (a.k.a. MoV1sS2gp1)
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer ovS2T1L-F
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    S1
  • Species
    Orthoreovirus T1L
  • Entrez Gene
    MoV1sS1gp2 (a.k.a. MoV1sS1gp2)
  • Entrez Gene
    MoV1sS1gp1 (a.k.a. MoV1sS1gp1)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer none
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    S4
  • Species
    Orthoreovirus T1L
  • Entrez Gene
    MoV1sS4gp1 (a.k.a. MoV1sS4gp1)
  • Promoter T7

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer ovS4T1L-R
  • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    M1
  • Species
    Orthoreovirus T1L
  • Entrez Gene
    M1 (a.k.a. MoV1sM1gp1)
  • Promoter T7

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer ovM1T1L-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-M1-S1-S2-S4T1L was a gift from Terence Dermody (Addgene plasmid # 33306 ; http://n2t.net/addgene:33306 ; RRID:Addgene_33306)

  • For your References section:

    An improved reverse genetics system for mammalian orthoreoviruses. Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS. Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29. 10.1016/j.virol.2009.11.037 PubMed 20042210
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