pLEW100v5-trunc
(Plasmid
#33346)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 33346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEM
- Backbone size w/o insert (bp) 4417
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Vector typeTrypanosoma brucei expression vector
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLuciferase truncated to allow cytoplasmic instead of peroxisomal (glycosomal) expression
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SpeciesFirefly
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Insert Size (bp)1632
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Mutation21bp of wt luciferase deleted
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer LucNrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is a derivative of Addgene plasmid 24011: pLEW100v5.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEW100v5-trunc was a gift from George Cross (Addgene plasmid # 33346 ; http://n2t.net/addgene:33346 ; RRID:Addgene_33346)
Map uploaded by the depositor.
