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G273D HA-dynamin 1 pUHD10-3
(Plasmid #34689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34689 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 34689-D50 50 μg of DNA in Tris buffer $495
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DNA 34689-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUHD10-3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dynamin 1
  • Species
    H. sapiens (human)
  • Mutation
    G273D
  • Entrez Gene
    DNM1 (a.k.a. DEE31, DNM, EIEE31)
  • Tag / Fusion Protein
    • HA (variant) (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G273D HA-dynamin 1 pUHD10-3 was a gift from Sandra Schmid (Addgene plasmid # 34689 ; http://n2t.net/addgene:34689 ; RRID:Addgene_34689)

  • For your References section:

    Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin. Damke H, Baba T, van der Bliek AM, Schmid SL. J Cell Biol. 1995 Oct;131(1):69-80. 10.1083/jcb.131.1.69 PubMed 7559787
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