p3xFLAG-THAP11-rescue
(Plasmid
#37001)
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Depositing Lab
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Depositing OrganizationNorthwestern University
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 37001-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 37001-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonep3xFLAG-CMV14
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 6300
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTHAP11
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SpeciesH. sapiens (human)
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MutationMutations were generated at codons 273-275 converting GAA GTG AAG to GAG GTC AAA and at codons 295-297 converting GAA CTG CGT to GAG CTC CGC
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Entrez GeneTHAP11 (a.k.a. HRIHFB2206, CTG-B43a, CTG-B45d, RONIN)
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV fwd
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 37001-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 37001-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3xFLAG-THAP11-rescue was a gift from Debu Chakravarti (Addgene plasmid # 37001 ; http://n2t.net/addgene:37001 ; RRID:Addgene_37001) -
For your References section:
A Transcriptional Regulatory Role of the THAP11-HCF-1 Complex in Colon Cancer Cell Function. Parker JB, Palchaudhuri S, Yin H, Wei J, Chakravarti D. Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27. 10.1128/MCB.06033-11 PubMed 22371484