UQ5871
(Bacterial strain #37170)

• Depositing Lab: Gary Roberts
• Publication: Gary Roberts unpublished plasmids (unpublished)

Backbone

• Vector backbone: pUX3009
• Modifications to backbone: pUX2996 with RcoM binding sites (d + e + f).

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. Coli UQ5853
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: coxM1′::lacZ reporter system with RcoM binding sites "d + e + f"

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: pRcoM1bs-R

Resource Information

• Supplemental Documents:
  • Strains for production of RcoMBx-1 proteins and assay of in vivo activity

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmid pUX3009 (pUX2996 based coxM1′::lacZ reporter system with RcoM binding sites "d + e + f").

Strain is CmR, pUX3007 is KanR and SpecR. Can be grown on Kan only.

For strain usage see attached pdf.