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Depositing Lab
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Depositing OrganizationNational Cancer Institute (NCI)
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37328 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 37328-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 37328-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonecustom
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameLuciferase
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SpeciesD. melanogaster (fly)
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Entrez Geneluciferase (a.k.a. C25G6.3)
- Promoter CMV
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameGFP
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SpeciesAequorea victoria
- Promoter SV40
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 37328-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 37328-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCLucf was a gift from John Schiller (Addgene plasmid # 37328 ; http://n2t.net/addgene:37328 ; RRID:Addgene_37328) -
For your References section:
Role of heparan sulfate in attachment to and infection of the murine female genital tract by human papillomavirus. Johnson KM, Kines RC, Roberts JN, Lowy DR, Schiller JT, Day PM. J Virol. 2009 Mar;83(5):2067-74. Epub 2008 Dec 10. 10.1128/JVI.02190-08 PubMed 19073722
Map uploaded by the depositor.
