CK2alpha V66A/I174A-HA (pJD23)
(Plasmid #37362)

• Depositing Lab: David Litchfield
• Publication: Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-88. Epub 2008 Feb 7.

Backbone

• Vector backbone: pRC/CMV
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5542
• Total vector size (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CK2α V66A/I174A
• Alt name: CK2alpha
• Alt name: Casein Kinase II alpha
• Species: H. sapiens (human)
• Insert Size (bp): 1470
• Mutation: V66A, I174A
• Entrez Gene: CSNK2A1 (a.k.a. CK2A1, CKII, Cka1, Cka2, OCNDS)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA X3 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

there is a 3bp deletion (AGG) in the MCS 131.