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CK2alpha V66A/I174A-HA (pJD23)
(Plasmid #37362)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37362 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRC/CMV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5542
  • Total vector size (bp) 7000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CK2α V66A/I174A
  • Alt name
    CK2alpha
  • Alt name
    Casein Kinase II alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1470
  • Mutation
    V66A, I174A
  • Entrez Gene
    CSNK2A1 (a.k.a. CK2A1, CKII, Cka1, Cka2, OCNDS)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA X3 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

there is a 3bp deletion (AGG) in the MCS 131.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CK2alpha V66A/I174A-HA (pJD23) was a gift from David Litchfield (Addgene plasmid # 37362 ; http://n2t.net/addgene:37362 ; RRID:Addgene_37362)
  • For your References section:

    An unbiased evaluation of CK2 inhibitors by chemoproteomics: characterization of inhibitor effects on CK2 and identification of novel inhibitor targets. Duncan JS, Gyenis L, Lenehan J, Bretner M, Graves LM, Haystead TA, Litchfield DW. Mol Cell Proteomics. 2008 Jun;7(6):1077-88. Epub 2008 Feb 7. 10.1074/mcp.M700559-MCP200 PubMed 18258654