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pEBB HA cIAP1
(Plasmid #38232)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38232 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 38232-D50 50 μg of DNA in Tris buffer $495
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DNA 38232-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEBB
  • Backbone manufacturer
    Baltimore Lab
  • Backbone size w/o insert (bp) 5350
  • Total vector size (bp) 7150
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cIAP1
  • Alt name
    BIRC2
  • Alt name
    hIAP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • GenBank ID
    NM_001166.4
  • Entrez Gene
    BIRC2 (a.k.a. API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1)
  • Promoter EF1a
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer EF1aF
  • 3′ sequencing primer JL44 (GAGGGGTGCAAGGACCCAG)
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBB HA cIAP1 was a gift from Colin Duckett (Addgene plasmid # 38232 ; http://n2t.net/addgene:38232 ; RRID:Addgene_38232)

  • For your References section:

    Two distinct signalling cascades target the NF-kappaB regulatory factor c-IAP1 for degradation. Csomos RA, Wright CW, Galban S, Oetjen KA, Duckett CS. Biochem J. 2009 Apr 28;420(1):83-91. 10.1042/BJ20082140 PubMed 19243308
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