FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)
(Plasmid
#38262)
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Depositing Lab
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Depositing OrganizationDuke University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 38262 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 38262-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 38262-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDNA 3.1 zeo-
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameβ-arrestin2
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Alt nameArrb2
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Alt nameBARRES
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SpeciesR. norvegicus (rat)
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MutationArg 233, Arg237, and Lys251 converted to Glutamines, stop codon replaced with HindIII restriction site to create kinase domain fusion
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GenBank IDNM_012911.1
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Entrez GeneArrb2 (a.k.a. BARRES)
- Promoter CMV
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Tags
/ Fusion Proteins
- PIP5K Iα core kinase domain (residues 18-399) (C terminal on insert)
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPoint mutations described by Dr. Jim Keen.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 38262-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 38262-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K) was a gift from Robert Lefkowitz (Addgene plasmid # 38262 ; http://n2t.net/addgene:38262 ; RRID:Addgene_38262) -
For your References section:
Beta-arrestin scaffolding of phosphatidylinositol 4-phosphate 5-kinase Ialpha promotes agonist-stimulated sequestration of the beta2-adrenergic receptor. Nelson CD, Kovacs JJ, Nobles KN, Whalen EJ, Lefkowitz RJ. J Biol Chem. 2008 Jul 25;283(30):21093-101. Epub 2008 Jun 5. 10.1074/jbc.M800431200 PubMed 18534983
