pAT206/Sept7shRNA
(Plasmid #38299)

• Depositing Lab: Matthew Krummel
• Publication: Tooley et al Nat Cell Biol. 2009 Jan;11(1):17-26. Epub 2008 Nov 30.

Backbone

• Vector backbone: pSilencer 2.0-U6
• Backbone manufacturer: Ambion
• Backbone size w/o insert (bp): 3130
• Total vector size (bp): 3183
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Septin 7
• Alt name: sept7KD2
• Alt name: Cdc10
• Alt name: E430034N22
• gRNA/shRNA sequence: GGATCCGTTTGACCAATTT
• Species: M. musculus (mouse)
• GenBank ID: AK078895 NC_000075.6
• Entrez Gene: Septin7 (a.k.a. Cdc10, E430034N22, Sept7)
• Promoter: Human U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: LKO1-5
• 3′ sequencing primer: M13pUC-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry