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pAct1IsGFP-1
(Plasmid #38775)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 38775 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 38775-D50 50 μg of DNA in Tris buffer $495
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DNA 38775-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pBlueSGFP
  • Total vector size (bp) 5335
  • Vector type
    Plant transformation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Actin promoter and intron
  • Alt name
    Act1
  • Species
    Oryza sativa
  • Insert Size (bp)
    1350
  • Tags / Fusion Proteins
    • sGFP (S65T) (C terminal on backbone)
    • nos 3' terminator (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • 3′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are several sequence discrepancies between Addgene's quality control sequence and the available NCBI reference sequence for actin promoter. These differences should not affect function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAct1IsGFP-1 was a gift from Peggy Lemaux (Addgene plasmid # 38775 ; http://n2t.net/addgene:38775 ; RRID:Addgene_38775)

  • For your References section:

    Production of transgenic tall fescue and red fescue plants by particle bombardment of mature seed-derived highly regenerative tissues. Cho MJ, Ha CD, Lemaux PG. Plant Cell Rep. 2000 Nov;19(11):1084-1089. doi: 10.1007/s002990000238. 10.1007/s002990000238 PubMed 30754774
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