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pTT3DestSPHis10
(Plasmid #39356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39356 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pTT3DestHis10
  • Backbone manufacturer
    Addgene plasmid #39354
  • Backbone size (bp) 8279
  • Modifications to backbone
    Vector pTT3DestSPHis10 was constructed by introducing the CD33 signal peptide sequence into pTT3DestHis10 at PmeI site.
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Tags / Fusion Proteins
    • CD33 signal peptide (N terminal on backbone)
    • 10xHis (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTT3DestSPHis10 was a gift from John McCafferty (Addgene plasmid # 39356)

  • For your References section:

    Multiplexed expression and screening for recombinant protein production in mammalian cells. Chapple SD, Crofts AM, Shadbolt SP, McCafferty J, Dyson MR. BMC Biotechnol. 2006 Dec 22;6:49. 10.1186/1472-6750-6-49 PubMed 17187663
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