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pT7-Venus-Emi1 (914)
(Plasmid #39854)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39854 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 39854-D50 50 μg of DNA in Tris buffer $495
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DNA 39854-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pT7-Venus-C1
  • Modifications to backbone
    T7 promoter was inserted downstream of the CMV promoter, and EYFP was replaced with Venus using AgeI and BsrGI. SacI and HindIII in MCS are no longer unique.
  • Vector type
    in vitro transcription
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Emi1
  • Species
    H. sapiens (human)
  • Entrez Gene
    FBXO5 (a.k.a. EMI1, FBX5, Fbxo31)
  • Promoter T7
  • Tag / Fusion Protein
    • Venus (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI/EcoRI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-Venus-Emi1 (914) was a gift from Jonathon Pines (Addgene plasmid # 39854 ; http://n2t.net/addgene:39854 ; RRID:Addgene_39854)

  • For your References section:

    Emi1 is needed to couple DNA replication with mitosis but does not regulate activation of the mitotic APC/C. Di Fiore B, Pines J. J Cell Biol. 2007 May 7;177(3):425-37. 10.1083/jcb.200611166 PubMed 17485488
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