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pCMV Myc rat SAPAP4
(Plasmid #40218)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40218 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 40218-D50 50 μg of DNA in Tris buffer $495
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DNA 40218-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV5 (modified)
  • Modifications to backbone
    pCMV5 was modified to include an N-terminal Myc tag
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SAPAP4
  • Species
    R. norvegicus (rat)
  • Mutation
    N834D
  • Entrez Gene
    Dlgap4 (a.k.a. DAP-4, Dap4, SAPAP4)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SmaI, XmaI, BamHI or XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3')
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV Myc rat SAPAP4 was a gift from Yutaka Hata & Yoshimi Takai (Addgene plasmid # 40218 ; http://n2t.net/addgene:40218 ; RRID:Addgene_40218)

  • For your References section:

    SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. Takeuchi M, Hata Y, Hirao K, Toyoda A, Irie M, Takai Y. J Biol Chem. 1997 May 2;272(18):11943-51. 10.1074/jbc.272.18.11943 PubMed 9115257
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