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(Plasmid #40439)


Item Catalog # Description Quantity Price (USD)
Plasmid 40439 Standard format: Plasmid sent in bacteria as agar stab 1 $65 *

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Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    aa1867_2161; L1916N, L1976A, I2049A, V2074A, L2092N
  • Entrez Gene
    LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
  • Promoter polH
  • Tags / Fusion Proteins
    • HisTev (N terminal on insert)
    • GST-Tev (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer DSP2466For:GAATTTCGTCGACAGATC
  • 3′ sequencing primer DSP2466Rev:AATAACCCGGATCAGCTT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LRRK2_1867_2161_L1916N_L1976A_I2049A_V2074A_L2092N was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 40439 ; ; RRID:Addgene_40439)