pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]
(Plasmid
#40806)
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Depositing Lab
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Depositing OrganizationUniversity of Colorado, Boulder
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 40806-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 40806-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMM9 from pMB1
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 6900
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Modifications to backbonepMB1: Blair et al. PNAS 77,3504 (1980)
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418) ; G418
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMAP2K2
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Alt nameMKK2
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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Mutationdelta(48-55) + S222D + S226D
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GenBank IDNM_030662
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Entrez GeneMAP2K2 (a.k.a. CFC4, MAPKK2, MEK2, MKK2, PRKMK2)
- Promoter MSV-LTR
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer MSV-LTR
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Kun-liang Guan provided gene, Dr. George Vande Woude provided vector backbone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 40806-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 40806-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D] was a gift from Natalie Ahn (Addgene plasmid # 40806 ; http://n2t.net/addgene:40806 ; RRID:Addgene_40806) -
For your References section:
Megakaryocytic differentiation induced by constitutive activation of mitogen-activated protein kinase kinase. Whalen AM, Galasinski SC, Shapiro PS, Nahreini TS, Ahn NG. Mol Cell Biol. 1997 Apr;17(4):1947-58. PubMed 9121442
Map uploaded by the depositor.
