pcDNA3.1 JellyOp
(Plasmid #41432)

• Depositing Lab: Robert Lucas
• Depositing Organization: University of Manchester
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Bailes et al PLoS One. 2012;7(1):e30774. Epub 2012 Jan 24.
• Citations: 1 Article

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5347
• Total vector size (bp): 6373
• Modifications to backbone: An HpaI recognition site was introduced 34 bases downstream of the CMV promoter region in the pcDNA3.1 backbone to enable cloning in of 5' blunted inserts (e.g. JellyOp).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Box jellyfish opsin
• Alt name: JellyOp
• Species: Carybdea rastonii
• Insert Size (bp): 1026
• GenBank ID: AB435549
• Promoter: CMV
• Tag / Fusion Protein:
  • 1D4 epitope at 3' end (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: M13 F
• 3′ sequencing primer: M13 R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This gene was synthesised by Genscript Corp at our request and provided in a puc57 cloning vector, from which our lab constructed this pcDNA3.1 JellyOp plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The original JellyOp gene from which we designed this coding region with a 1D4 fusion was sequenced by Koyanagi et al (2008). PNAS 105:15576-15580.

DNA (Catalog # 41432-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 41432-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.