pSKDuet20
(Plasmid
#41694)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSFDuet-1
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Backbone manufacturernovagen
- Total vector size (bp) 4989
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Modifications to backboneremoved the second MCS of pRSFDuet-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMjaTFIIB intein
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SpeciesMethanococcus jannaschii
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Insert Size (bp)1423
- Promoter T7
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Tags
/ Fusion Proteins
- His-tag (N terminal on insert)
- GB1 (N terminal on insert)
- GB1 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSKDuet20 was a gift from Hideo Iwai (Addgene plasmid # 41694 ; http://n2t.net/addgene:41694 ; RRID:Addgene_41694) -
For your References section:
Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. Ellila S, Jurvansuu JM, Iwai H. FEBS Lett. 2011 Oct 10. 10.1016/j.febslet.2011.10.005 PubMed 22001202