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Nuc5-.dnaG.Q576A.tolC-
(Bacterial strain #41699)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 41699 Bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Nuc5-.dnaG.Q576A
  • Growth instructions
    Do not grow above 34 C because lambda red will begin to have leaky expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E. coli MG1655 ΔmutS::cat Δ(ybhB-bioAB)::[λcI857 N(cro-ea59)::tetR-bla] xonA-, recJ-,xseA-, exoX- and redα- dnaG.Q576A tolC-

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nuc5-.dnaG.Q576A.tolC- was a gift from George Church (Addgene plasmid # 41699)
  • For your References section:

    Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering. Lajoie MJ, Gregg CJ, Mosberg JA, Washington GC, Church GM. Nucleic Acids Res. 2012 Aug 16. 10.1093/nar/gks751 PubMed 22904085