pNH053
(Plasmid
#42159)
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Depositing Lab
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Depositing OrganizationKing's College London
Located in United Kingdom of Great Britain and Northern Ireland -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42159 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 42159-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 42159-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCC1Fos
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Backbone manufacturerEipcentre
- Backbone size w/o insert (bp) 8353
- Total vector size (bp) 10764
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Tetracycline, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Growth instructionspCC1Fos-based 'CopyControl' construct enabling transient induction of copy-number
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemCherry-RT-mCherry
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Insert Size (bp)2411
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
DNA (Catalog # 42159-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 42159-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNH053 was a gift from Colin Dolphin (Addgene plasmid # 42159 ; http://n2t.net/addgene:42159 ; RRID:Addgene_42159) -
For your References section:
A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT. BMC Biotechnol. 2013 Jan 3;13(1):1. 10.1186/1472-6750-13-1 PubMed 23281894
Map uploaded by the depositor.
Map uploaded by the depositor.
