|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42197||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFrizzled 4
Alt namefrizzled homolog 4
SpeciesM. musculus (mouse)
GenBank IDNM_008055.4 NP_032081.3
Entrez GeneFzd4 (a.k.a. Fz4)
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N, SV40pA-R (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
Fz4-GFP was generated by fusing EGFP (Clontech) at its C-terminus.
Alternate plasmid names: pEGFPN2 mFz4; pEGFP-N2-Frizzled4
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Fz4-GFP was a gift from Robert Lefkowitz & Jeremy Nathans (Addgene plasmid # 42197 ; http://n2t.net/addgene:42197 ; RRID:Addgene_42197)
For your References section:Dishevelled 2 recruits beta-arrestin 2 to mediate Wnt5A-stimulated endocytosis of Frizzled 4. Chen W, ten Berge D, Brown J, Ahn S, Hu LA, Miller WE, Caron MG, Barak LS, Nusse R, Lefkowitz RJ. Science. 2003 Sep 5;301(5638):1391-4. 10.1126/science.1082808 PubMed 12958364
Map uploaded by the depositor.