pEGFP VAMP7 (1-220)
(Plasmid #42316)

• Depositing Lab: Thierry Galli
• Depositing Organization: Delegation INSERM Paris 7
  Located in France
• Publication: Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.
• Citations: 14 Articles

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VAMP7 (1-220)
• Alt name: VAMP7
• Alt name: vesicle-associated membrane protein 7
• Alt name: TI-VAMP/Synaptobrevin-like (Sybl 1)
• Species: H. sapiens (human)
• GenBank ID: NM_005638.5 NP_005629.1
• Entrez Gene: VAMP7 (a.k.a. SYBL1, TI-VAMP, TIVAMP, VAMP-7)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C; CMV-F
• 3′ sequencing primer: SV40-pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alternate plasmid name: Full-length TI-VAMP (TIVAMP)

For production of NH2-terminal GFP fusion proteins, the distinct cDNAs were cloned into the pEGFP-C3 vector (CLONTECH Laboratories, Inc.). Full-length TI-VAMP (TIVAMP), NH2-terminal domain-TIVAMP (Nter-TIVAMP, from M to N120), cytosolic domain-TIVAMP (Cyt-TIVAMP, from M to K188), and ΔNH2-terminal domain-TIVAMP (ΔNter-TIVAMP, from M102 to the end), were obtained by PCR using standard procedures and the following sets of oligonucleotides:

5′-ATGGCGATTCTTTTTGCTGTTGTTGCC-3′
and
5′-CTATTTCTTCACACAGCTTGGCCATGT-3′
for TIVAMP;

5′-ATGGCGATTCTTTTTGCTGTTGTTGCC-3′
and
5′-CTTATTCTCAGAGTGATGCTTCAG-CTG-3′
for Nter-TIVAMP;

5′-ATGGCGATTCTTTTTGCTGTTGTTGCC-3′
and
5′-ATCCTACTTGAGGTTCTTCATACACATGGCTC
for Cyt-TIVAMP;

5′-ATGAATAGCGAGTTCTCAAGTGTCTTA-3′
and
5′-CTATTTCTTCACACAGCTTGGCCATGT-3′
for ΔNter-TIVAMP.

DNA (Catalog # 42316-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 42316-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.