pcDNA 3.1/V5-His TOPO TA mouse Mns1
(Plasmid
#42899)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA 3.1/V5-His TOPO TA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMns1
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Alt namemeiosis-specific nuclear structural protein 1
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Alt nameAW546487
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SpeciesM. musculus (mouse)
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GenBank IDNM_008613.3 NP_032639.1
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Entrez GeneMns1 (a.k.a. AW546487)
- Promoter CMV
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Tags
/ Fusion Proteins
- V5 (C terminal on backbone)
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The cDNA encoding full-length mouse Mns1, complete CDS with Kozak sequence, was cloned into pcDNA 3.1/V5-His TOPO TA (Invitrogen) for V5 tagging.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA 3.1/V5-His TOPO TA mouse Mns1 was a gift from Jeremy Wang (Addgene plasmid # 42899 ; http://n2t.net/addgene:42899 ; RRID:Addgene_42899) -
For your References section:
MNS1 is essential for spermiogenesis and motile ciliary functions in mice. Zhou J, Yang F, Leu NA, Wang PJ. PLoS Genet. 2012;8(3):e1002516. doi: 10.1371/journal.pgen.1002516. Epub 2012 Mar 1. 10.1371/journal.pgen.1002516 PubMed 22396656