pNES-VL12.3
(Plasmid #43862)

• Depositing Lab: Dane Wittrup
• Publication: Colby et al Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17616-21. Epub 2004 Dec 14.

Backbone

• Vector backbone: pcDNA3.1(-)Zeo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VL12.3
• Species: Synthetic
• Insert Size (bp): 366
• GenBank ID: AY818434.1
• Promoter: CMV
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 Fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry