pcDNA-HA-hTRAF3 1-448
(Plasmid
#44033)
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Depositing Lab
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Depositing OrganizationPennsylvania State University, College of Medicine
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44033 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 44033-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 44033-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA-HA
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Backbone manufacturerShao-Cong Sun
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTNF receptor-associated factor 3
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Alt nameTRAF3
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SpeciesH. sapiens (human)
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MutationContains amino acids 1-448; V274D and S393G
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Entrez GeneTRAF3 (a.k.a. CAP-1, CAP1, CD40bp, CRAF1, IIAE5, LAP1, RNF118)
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Tag
/ Fusion Protein
- 1xHA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pCDNA-HA backbone: Inserted influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) with the translation initiation codon (ATG) into pcDNA3.1 vector (Invitrogen); described in Harhaj, et al. (1999) JBC, 274, 22911–22914.
Please note that the hTRAF3 insert in this plasmid contains V274D and S393G mutations when compared to GenBank entry NP_663777.1
DNA (Catalog # 44033-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 44033-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-HA-hTRAF3 1-448 was a gift from Shao-Cong Sun (Addgene plasmid # 44033 ; http://n2t.net/addgene:44033 ; RRID:Addgene_44033) -
For your References section:
Regulation of the NF-kappaB-inducing kinase by tumor necrosis factor receptor-associated factor 3-induced degradation. Liao G, Zhang M, Harhaj EW, Sun SC. J Biol Chem. 2004 Jun 18;279(25):26243-50. Epub 2004 Apr 14. 10.1074/jbc.M403286200 PubMed 15084608
Map uploaded by the depositor.
