pMpGWB102-Citrine-PTS1
(Plasmid #44592)

• Purpose: Binary vectors for expression of Citrine fluorescent protein with a peroxisome localization signal in plants
• Depositing Lab: Yutaka Kodama
• Publication: Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10.1111/pce.12085.

Backbone

• Vector backbone: pMpGWB102
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 10404
• Total vector size (bp): 11130
• Modifications to backbone: These PCR-amplified DNA fragments of Citrine-PTS1 were cloned into a pDONR207 plasmid by BP reaction (Invitrogen). The resulting plasmids were mixed with pMpGWB102 vector, and LR reaction (Invitrogen) was performed to generate pMpGWB102-Citrine-PTS1.
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Citrine-PTS1
• Species: Synthetic
• Insert Size (bp): 729
• Promoter: CaMV 35S promoter
• Tag / Fusion Protein:
  • PTS1 (peroxisomal targeting signal type 1) (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATGGTGAGCAAGGGCGAG
• 3′ sequencing primer: GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACAACTTGGACTTGTACAG CTCGTCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry