pIRESN2 H2B-GFP-uvr8at(2x)
(Plasmid
#44970)
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Depositing Lab
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Depositing OrganizationUniversity of Geneva
Located in Switzerland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 44970-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 44970-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepIRESneo2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5308
- Total vector size (bp) 9133
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B-GFP-UVR8(2x)
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Alt namehistone H2B
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Alt nameUV-B resistance 8
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SpeciesH. sapiens (human), A. thaliana (mustard weed)
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Insert Size (bp)3825
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Mutationtandem repeat of UVR8 (both have Methionine 1 deleted) linked by a flexible linker: GDSGDGKGGKGSGDGSGGDGSG
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GenBank IDNM_021058 NM_125781.3
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Entrez GeneUVR8 (a.k.a. AT5G63860, MGI19.7, MGI19_7, UVB-RESISTANCE 8)
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Entrez GeneH2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
- Promoter pCMV IE
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Tag
/ Fusion Protein
- internal GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AflII (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer IRES-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 44970-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 44970-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRESN2 H2B-GFP-uvr8at(2x) was a gift from Thanos Halazonetis (Addgene plasmid # 44970 ; http://n2t.net/addgene:44970 ; RRID:Addgene_44970) -
For your References section:
Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells. Crefcoeur RP, Yin R, Ulm R, Halazonetis TD. Nat Commun. 2013;4:1779. doi: 10.1038/ncomms2800. 10.1038/ncomms2800 PubMed 23653191
