|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45402||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8145
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Growth Strain(s)DH1 w/ AcrAB knocked out
Copy numberLow Copy
Insert Size (bp)4490
- Promoter LacUV5
/ Fusion Protein
- His (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CAA AAG ATC TTT TAA GAA GGA GAT ATA CAT ATG GGC ATC CTG AAC GGA CGG TCG
- 3′ sequencing primer CAG TGG TGA TGG TGA TGA TGG TCG TCC TCG CGC CGG CGT TGC G (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byATCC BAA-1303
Terms and Licenses
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBbA5k-EPL4 was a gift from Aindrila Mukhopadhyay (Addgene plasmid # 45402 ; http://n2t.net/addgene:45402 ; RRID:Addgene_45402)
For your References section:Engineering microbial biofuel tolerance and export using efflux pumps. Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi MZ, Mukhopadhyay A. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. 10.1038/msb.2011.21 PubMed 21556065