pcDNA3.1 AT1R DRY-AAY
(Plasmid #45759)

• Depositing Labs: Laszlo Hunyady, Robert Lefkowitz
• Depositing Organization: Semmelweis University
  Located in Hungary
• Publication: Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.
• Citations: 1 Article

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AT1R DRY-AAY
• Alt name: AT1AR
• Alt name: angiotensin II receptor, type 1a
• Species: R. norvegicus (rat)
• Mutation: changed amino acids 125-126 from DR to AA
• Entrez Gene: Agtr1a (a.k.a. AT1, AT1A, AT1R, Agtr1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F; T7
• 3′ sequencing primer: BGHrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Rat vascular smooth muscle AT1A receptor cDNA from Dr. K. E. Bernstein (Emory University, Atlanta, GA).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The cDNA of the rat vascular smooth muscle AT1A receptor was donated by Dr. K. E. Bernstein (Emory University, Atlanta, GA). Mutations in the rat AT1A receptor were performed with the Mutagene kit(Bio-Rad Laboratories, Inc., Hercules, CA)

N4D mutation is present to prevent glycosylation at this consensus site and steric hindrance of antibody binding.

DNA (Catalog # 45759-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 45759-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.