IL2R/hE-cadherin-cytotail
(Plasmid #45773)

• Depositing Lab: Barry Gumbiner
• Depositing Organization: Memorial Sloan-Kettering Cancer Center
  Located in United States of America
• Publication: Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.
• Citations: 2 Articles

Backbone

• Vector backbone: pCMV
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IL2R/hE-cadherin-cytotail
• Alt name: Interleukin-2 receptor α subunit extracellular and transmembrane domains
• Alt name: E-cadherin cytoplasmic domain
• Species: H. sapiens (human)
• Mutation: Interleukin-2 receptor α subunit extracellular and transmembrane domains fused to E-cadherin cytoplasmic domain
• Entrez Gene: IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
• Entrez Gene: CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F; T7
• 3′ sequencing primer: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human E-cadherin partial cDNA provided by David Rimm (Yale University, New Haven, CT). IL2R-1 α subunit cDNA from pCMV-IL2R-1 from Susan LaFlamme (Albany Medical College, Albany, NY), originally from Anthony Giordano (Giordano et al ., 1991)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This IL2R/E-cadherin cytoplasmic chimera fuses the extracellular and transmembrane domains of the interleukin-2 receptor α subunit to the cytoplasmic domain of E-cadherin. This construct can bind β-catenin but cannot engage in homophilic adhesive activity.

DNA (Catalog # 45773-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 45773-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.