Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #48740)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 48740 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 459
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
    C. elegans UNC-60B cDNA
  • Alt name
    C. elegans ADF/cofilin
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
  • Entrez Gene
    unc-60 (a.k.a. CELE_C38C3.5)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see the insert sequence provided by the depositing laboratory and Figure 1 from the associated publication (Ono S and Benian GM, JBC 1998) listed below for information on the UNC-60 isoform in this plasmid. This plasmid encodes the UNC-60B isoform as described in the publication and now referred to as UNC-60C by NCBI and Wormbase (GenBank reference sequence NP_503427.2).

The original nomenclature of UNC-60A and UNC-60B was defined in the publication "Genetic organization of the unc-60 region in Caenorhabditis elegans." McKim KS, Heschl MF, Rosenbluth RE, Baillie DL. Genetics. 1988 Jan;118(1):49-59.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-UNC60B was a gift from Shoichiro Ono (Addgene plasmid # 48740 ; ; RRID:Addgene_48740)
  • For your References section:

    Two Caenorhabditis elegans actin depolymerizing factor/cofilin proteins, encoded by the unc-60 gene, differentially regulate actin filament dynamics. Ono S, Benian GM. J Biol Chem. 1998 Feb 6;273(6):3778-83. 10.1074/jbc.273.6.3778 PubMed 9452511