pET-UNC60B
(Plasmid #48740)

• Purpose: Bacterial expression of UNC-60B (a C. elegans ADF/cofilin)
• Depositing Lab: Shoichiro Ono
• Publication: Ono et al J Biol Chem. 1998 Feb 6;273(6):3778-83.

Backbone

• Vector backbone: pET-3d
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 459
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C. elegans UNC-60B cDNA
• Alt name: C. elegans ADF/cofilin
• Species: C. elegans (nematode)
• Insert Size (bp): 459
• Entrez Gene: unc-60 (a.k.a. CELE_C38C3.5)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the insert sequence provided by the depositing laboratory and Figure 1 from the associated publication (Ono S and Benian GM, JBC 1998) listed below for information on the UNC-60 isoform in this plasmid. This plasmid encodes the UNC-60B isoform as described in the publication and now referred to as UNC-60C by NCBI and Wormbase (GenBank reference sequence NP_503427.2).

The original nomenclature of UNC-60A and UNC-60B was defined in the publication "Genetic organization of the unc-60 region in Caenorhabditis elegans." McKim KS, Heschl MF, Rosenbluth RE, Baillie DL. Genetics. 1988 Jan;118(1):49-59. https://www.ncbi.nlm.nih.gov/pubmed/8608931