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pTA7002-avrPto
(Plasmid #49156)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49156 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 49156-D50 50 μg of DNA in Tris buffer $495
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DNA 49156-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTA7002
  • Backbone manufacturer
    Nam-Hai Chua
  • Vector type
    Plant transformation binary vector
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Please note: a binary vector-compatible Agrobacterium strain (such as GV3850) will have to be used prior to plant transformation. Plasmid should be grown at 30C after transformation to Agrobacterium (37C is ok for E. coli).
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    avrPto
  • Alt name
    avrPto1
  • Alt name
    avrPto1DC3000
  • Species
    Pseudomonas syringae pv. tomato DC3000
  • Insert Size (bp)
    495
  • GenBank ID
    NC_004578.1
  • Promoter dexamethasone inducible

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SpeI (unknown if destroyed)
  • 5′ sequencing primer CCGCTCGAGACCATGGGAAATATATGTGTC
  • 3′ sequencing primer GACTAGTTCATTGCCAGTTACGGTACG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

An efficient Agrobacterium-mediated transient transformation of Arabidopsis.
K. Tsuda et al. Plant J. 2012 Feb;69(4):713-9

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTA7002-avrPto was a gift from Sheng Yang He (Addgene plasmid # 49156 ; http://n2t.net/addgene:49156 ; RRID:Addgene_49156)

  • For your References section:

    A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants. Hauck P, Thilmony R, He SY. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8577-82. Epub 2003 Jun 19. 10.1073/pnas.1431173100 PubMed 12817082
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