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pOG2002
(Plasmid #49514)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49514 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD95.77
  • Backbone manufacturer
    Andrew Fire lab
  • Vector type
    Worm Expression
  • Promoter hsp-16.2
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on backbone)
    • 3xFLAG (N terminal on backbone)
    • FokI-AS (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

725 bp of unc54 3'UTR was used for C. elegans expression.
mutations in pthA: delta 1-152, delta 299-875, delta 949-1163
mutations in ZNF-fokI: E484A and R487S

This vector and Addgene plasmid # 49516 are used for insertion of custom repeats which can be assembled by the unit assembly method.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOG2002 was a gift from Guangshuo Ou (Addgene plasmid # 49514 ; http://n2t.net/addgene:49514 ; RRID:Addgene_49514)
  • For your References section:

    Conditional targeted genome editing using somatically expressed TALENs in C. elegans. Cheng Z, Yi P, Wang X, Chai Y, Feng G, Yang Y, Liang X, Zhu Z, Li W, Ou G. Nat Biotechnol. 2013 Oct;31(10):934-7. doi: 10.1038/nbt.2674. Epub 2013 Aug 18. 10.1038/nbt.2674 PubMed 23955274