Purposeexpresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50050||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 7400
Modifications to backboneThe vector is modified for LIC cloning, but the insert can also be extracted using 5' Nhe I/sal I/BsiW1, and 3' Spe I, Asc I/BssH II.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Alt nameTRAP Homolog
Insert Size (bp)2000
MutationS158A, N357S, N463S, N470D
- Promoter CMV
/ Fusion Proteins
- TEV (C terminal on insert)
- SBP (C terminal on insert)
- HIS (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDr. David Sibley Washington University School of Medicine
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that Addgene has not been unable to verify the N357S, N463S, N470D mutations in the MIC2 sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MIC2 was a gift from Timothy Springer (Addgene plasmid # 50050 ; http://n2t.net/addgene:50050 ; RRID:Addgene_50050)