PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50386||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Alt namepantothenate kinase
- Promoter T7
/ Fusion Protein
- 6XHis-tag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a-Ec.coaA was a gift from Erick Strauss (Addgene plasmid # 50386 ; http://n2t.net/addgene:50386 ; RRID:Addgene_50386)
For your References section:Characterization of a new pantothenate kinase isoform from Helicobacter pylori. Brand LA, Strauss E. J Biol Chem. 2005 May 27;280(21):20185-8. Epub 2005 Mar 28. 10.1074/jbc.C500044200 PubMed 15795230