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(Plasmid #50976)


Item Catalog # Description Quantity Price (USD)
Plasmid 50976 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4599
  • Total vector size (bp) 6390
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Regular growing condition.
  • Copy number


  • Gene/Insert name
  • Alt name
    mammalian codon-optimzed ChD-citrine
  • Insert Size (bp)
  • Mutation
    ChD gene is mammalian codon optimized
  • GenBank ID
  • Promoter human synapsin promoter
  • Tag / Fusion Protein
    • citrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

WPRE is inserted after the citrine before SV40 polyA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-oChD-citrine was a gift from Roger Tsien (Addgene plasmid # 50976 ; ; RRID:Addgene_50976)
  • For your References section:

    Characterization of engineered channelrhodopsin variants with improved properties and kinetics. Lin JY, Lin MZ, Steinbach P, Tsien RY. Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034. 10.1016/j.bpj.2008.11.034 PubMed 19254539