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pET-Ce25
(Plasmid #51296)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51296 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET32a+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 7693
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    arginyl-tRNA synthetase
  • Alt name
    RARS
  • Species
    Canavalia ensiformis (jack bean)
  • Insert Size (bp)
    1791
  • Mutation
    GTT (Val) insertion after initiation codon to permit NcoI cloning
  • GenBank ID
    AM950325.1
  • Promoter T7
  • Tags / Fusion Proteins
    • Thioredoxin (N terminal on backbone)
    • His Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For induction, use BL21CodonPlus, cool and grow at 23°C

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Ce25 was a gift from Gabor Igloi (Addgene plasmid # 51296 ; http://n2t.net/addgene:51296 ; RRID:Addgene_51296)
  • For your References section:

    Expression and properties of arginyl-tRNA synthetase from jack bean (Canavalia ensiformis). Hogg J, Schiefermayr E, Schiltz E, Igloi GL. Protein Expr Purif. 2008 Oct;61(2):163-7. doi: 10.1016/j.pep.2008.05.008. Epub 2008 May 23. 10.1016/j.pep.2008.05.008 PubMed 18562210