Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pUC119-SA12
(Plasmid #51995)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51995 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC119
  • Backbone size w/o insert (bp) 3162
  • Total vector size (bp) 8392
  • Modifications to backbone
    SA12 was cloned into the unique PstI site of pUC119.
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SA12
  • Alt name
    Simian virus 12
  • Alt name
    Simian agent 12
  • Species
    Simian virus 12
  • Insert Size (bp)
    5230
  • GenBank ID
    AY614708.1
  • Promoter none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC119-SA12 was a gift from James Pipas (Addgene plasmid # 51995 ; http://n2t.net/addgene:51995 ; RRID:Addgene_51995)
  • For your References section:

    Complete nucleotide sequence of polyomavirus SA12. Cantalupo P, Doering A, Sullivan CS, Pal A, Peden KW, Lewis AM, Pipas JM. J Virol. 2005 Oct;79(20):13094-104. 10.1128/JVI.79.20.13094-13104.2005 PubMed 16189011