Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pUAST-HDAC4 WT
(Plasmid #52977)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52977 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8904
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HDAC4
  • Species
    D. melanogaster (fly)
  • Mutation
    *
  • Promoter Hsp70
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pCasper-hs
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    HDAC4 from the DGRC

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note that there are some mismatches between the HDAC4 in this plasmid and the top BLAST hits for this gene in GenBank. The depositor maintains that the clone in this plasmid is what was acquired from the DGRC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST-HDAC4 WT was a gift from Marc Montminy & Biao Wang (Addgene plasmid # 52977 ; http://n2t.net/addgene:52977 ; RRID:Addgene_52977)
  • For your References section:

    A hormone-dependent module regulating energy balance. Wang B, Moya N, Niessen S, Hoover H, Mihaylova MM, Shaw RJ, Yates JR 3rd, Fischer WH, Thomas JB, Montminy M. Cell. 2011 May 13;145(4):596-606. doi: 10.1016/j.cell.2011.04.013. 10.1016/j.cell.2011.04.013 PubMed 21565616