pET-Gm1
(Plasmid #53528)

• Purpose: Expresses a plant arginyl-tRNA synthetase in E.coli
• Depositing Lab: Gabor Igloi
• Publication: Igloi et al FEBS J. 2009 Mar;276(5):1307-18. doi: 10.1111/j.1742-4658.2009.06866.x.

Backbone

• Vector backbone: pET32a+
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5900
• Total vector size (bp): 7696
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For induction, grow at 23°C. Expression results largely in insoluble protein with a low yield of soluble, active enzyme
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: arginyl-tRNA synthetase
• Alt name: RARS
• Species: Glycin max (soybean)
• Insert Size (bp): 1794
• GenBank ID: FM209045
• Promoter: T7
• Tags / Fusion Proteins:
  • Thioredoxin (N terminal on backbone)
  • His Tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • Expression and purification

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry