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pET-Cb2
(Plasmid #53529)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53529 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET32a+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 7580
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mitochondrial arginyl-tRNA synthetase
  • Alt name
    RARS2
  • Species
    Caryedes brasiliensis (Coleoptera)
  • Insert Size (bp)
    1692
  • Mutation
    changed Ser4 to Ala4 to permit NcoI cloning
  • GenBank ID
    KF419296
  • Promoter T7
  • Tags / Fusion Proteins
    • Thioredoxin (N terminal on backbone)
    • His Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

For expression grow in E. coli BL21CodonPlus at 23°C. The protein is expressed largely in an insoluble form but small amounts of active enzyme may be recovered.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Cb2 was a gift from Gabor Igloi (Addgene plasmid # 53529 ; http://n2t.net/addgene:53529 ; RRID:Addgene_53529)
  • For your References section:

    Amino acid discrimination by the nuclear encoded mitochondrial arginyl-tRNA synthetase of the larva of a bruchid beetle (Caryedes brasiliensis) from northwestern Costa Rica. Leisinger AK, Janzen DH, Hallwachs W, Igloi GL. Insect Biochem Mol Biol. 2013 Dec;43(12):1172-80. PubMed 24446543