PurposeLocalization: Golgi TGN, Excitation: 580, Emission: 608
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||56133||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Mutationaa 1-45 of SiT
Entrez GeneST6GAL1 (a.k.a. SIAT1, ST6GalI, ST6N)
- Promoter CMV
/ Fusion Protein
- FusionRed (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
mKate2.7C = FusionRed. Excitation = 580; Emission = 608
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FusionRed-Sit-N-15 was a gift from Michael Davidson (Addgene plasmid # 56133 ; http://n2t.net/addgene:56133 ; RRID:Addgene_56133)
For your References section:A monomeric red fluorescent protein with low cytotoxicity. Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM, Shcherbo D. Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208. 10.1038/ncomms2208 PubMed 23149748