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pLVX-FADD-DD
(Plasmid #58263)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58263 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLVX-IRES-Hygro
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8519
  • Total vector size (bp) 8924
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FADD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    405
  • Mutation
    version of FADD (FADD-DD) that contains the FADD death domain (aa30-124), but lacks the death effector domain that binds to caspase-8
  • GenBank ID
    NM_003824.3
  • Entrez Gene
    FADD (a.k.a. GIG3, MORT1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GCAGAGCTCGTTTAGTGAACC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Andrew M. Thorburn Professor and Vice Chair, Dept. of Pharmacology University of Colorado Denver Department of Pharmacology
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-FADD-DD was a gift from Joan Massague (Addgene plasmid # 58263 ; http://n2t.net/addgene:58263 ; RRID:Addgene_58263)
  • For your References section:

    Serpins promote cancer cell survival and vascular co-option in brain metastasis. Valiente M, Obenauf AC, Jin X, Chen Q, Zhang XH, Lee DJ, Chaft JE, Kris MG, Huse JT, Brogi E, Massague J. Cell. 2014 Feb 27;156(5):1002-16. doi: 10.1016/j.cell.2014.01.040. 10.1016/j.cell.2014.01.040 PubMed 24581498