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pCMV-CIB1-mRFP1-MP
(Plasmid #58367)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58367 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 58367-D50 50 μg of DNA in Tris buffer $495
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DNA 58367-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pmCerulean-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4742
  • Total vector size (bp) 5687
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CIB1[1-147]
  • Alt name
    cryptochrome-interacting basic-helix-loop-helix 1
  • Alt name
    T4L20.110
  • Alt name
    T4L20_110
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    513
  • GenBank ID
    829604
  • Entrez Gene
    CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mRFP1 (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Camk2a[315-478]
  • Alt name
    calcium/calmodulin-dependent protein kinase II alpha
  • Alt name
    PKCCD
  • Alt name
    PK2CDD
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    498
  • GenBank ID
    25400
  • Entrez Gene
    Camk2a (a.k.a. PK2CDD, PKCCD)
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-CIB1-mRFP1-MP was a gift from Won Do Heo (Addgene plasmid # 58367 ; http://n2t.net/addgene:58367 ; RRID:Addgene_58367)

  • For your References section:

    Reversible protein inactivation by optogenetic trapping in cells. Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD. Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 2014 May 4. 10.1038/nmeth.2940 PubMed 24793453
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