PurposeLentiviral expression vector: Neuroligin 1 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||59339||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8777
- Total vector size (bp) 8831
Modifications to backboneGFP expression is driven by CAG-promoter (instead of CMV-promoter)
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsGrowth in standard bacterial strains (e.g. DH5alpha) at 37°C is possible, however, growth in SURE cells (37°C) or Stbl3 cells (30°C) is recommended to reduce the risk of recombination.
Copy numberHigh Copy
Gene/Insert nameNeuroligin 1 shRNA (rat)
SpeciesR. norvegicus (rat)
Insert Size (bp)54
- Promoter mouse U6
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer mU6-F (5'-CAGCACAAAAGGAAACTCACC-3'); mU6-F2 (5'-ACAGACTTGTGGGAGAAGC-3') (Common Sequencing Primers)
Alt nameenhanced green fluorescent protein
Insert Size (bp)720
- Promoter CAG
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3') (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Suitable for knockdown of rat Nlgn1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenLox_U6 Nlgn1 was a gift from Peter Scheiffele (Addgene plasmid # 59339 ; http://n2t.net/addgene:59339 ; RRID:Addgene_59339)
For your References section:Control of excitatory and inhibitory synapse formation by neuroligins. Chih B, Engelman H, Scheiffele P. Science. 2005 Feb 25;307(5713):1324-8. Epub 2005 Jan 27. 10.1126/science.1107470 PubMed 15681343