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pLenLox_U6 Nlgn1
(Plasmid #59339)


Item Catalog # Description Quantity Price (USD)
Plasmid 59339 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 8777
  • Total vector size (bp) 8831
  • Modifications to backbone
    GFP expression is driven by CAG-promoter (instead of CMV-promoter)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Growth in standard bacterial strains (e.g. DH5alpha) at 37°C is possible, however, growth in SURE cells (37°C) or Stbl3 cells (30°C) is recommended to reduce the risk of recombination.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Neuroligin 1 shRNA (rat)
  • Alt name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
  • Promoter mouse U6

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer mU6-F (5'-CAGCACAAAAGGAAACTCACC-3'); mU6-F2 (5'-ACAGACTTGTGGGAGAAGC-3')
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Alt name
    enhanced green fluorescent protein
  • Insert Size (bp)
  • Promoter CAG

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Suitable for knockdown of rat Nlgn1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenLox_U6 Nlgn1 was a gift from Peter Scheiffele (Addgene plasmid # 59339 ; ; RRID:Addgene_59339)
  • For your References section:

    Control of excitatory and inhibitory synapse formation by neuroligins. Chih B, Engelman H, Scheiffele P. Science. 2005 Feb 25;307(5713):1324-8. Epub 2005 Jan 27. 10.1126/science.1107470 PubMed 15681343