pSAG1::CAS9-U6::sg290860-6 Sequences (6)

Addgene-Verified Sequences:

Partial (5)

Depositor Sequences:

Full (1)

Depositor Full Sequences (1)

Sequences from the depositing laboratory may be predicted or based on Sanger or NGS data. Addgene sequencing results may differ from the depositor-provided sequence.

Analyze Sequence
Sequence > pSAG1::CAS9-U6::sg290860-6 caagtaagcagaagcacgctgtatttccgggagggtgcgacgagacaaagtgcgcgagttgaaatcgtcg tggggacgattgcaccgcggccacatgttggagacactgaggacacacgggaaacgcgaaagatttcaaa ttaacgtacccaagcgcgaaagcttgcgcagcatacactcgaagcgaacatcccgaaccatcgagaggcg gagagcgataagtctttcacgctgcgaagtgttgcgacggctgcgccgctgcactgtgaattgggcgcca atattgcatcctaggcctgacgcgcctcctgcagaacgcgagacactgggatatgtagagccaaggggga aaccttcgaactctcgaatgtcttctctgacaagaatcatatttccatcagttctgtcagattttcaaat ggcgacctgcagaggcctgcttcctccctgtgcgctcttcgaaggggctttctgtcgcgcagggtcacct cgtccccgaagggggtgtttgccttctggtaaatggggatgtcaagttattggtctgacagcgatgtggt tttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaagtggcaccgagtcgg tgcttttttgagctccagcttttgttccctttagtgagggttaatttcgagcttggcgtaatcatggtca tagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagt gtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttcca gtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtatt gggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaa aggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccct gacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccagg cgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgc ctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtc gttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaact atcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattag cagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagg acagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccg gcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaagg atctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaaggg attttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaat caatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctc agcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggag ggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcag caataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtc tattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccatt gctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaa ggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcag aagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgcca tccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgac cgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcat cattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaa cccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacag gaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttt tcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaa aataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatatttt gttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatc ccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactat taaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaacc atcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccc cgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgg gcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgcc gctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctct tcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggtttt cccagtcacgacgttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaattggg taccgggccccccctcgaggtcgacggtatcgataagcttttacatccgttgccttttccacggtccgtg atttcatgtgcgtgcagcttcaaagactggtcgttgcgactaataagactgcagtgacaggtcgaatggt gggcaccttgctgatgactatctactgcaaagtctgagacaacgaacgaaacttcccacacgaggcattt gaaactgacggtgtctaggtaatatgcactgcaagacacggtactggggcctcgctgaattaggggccga tctcgttgccctatcagtgctcacagtgccgcaacgtaacaccagggcaggttcttgacagtggcaacaa tgtgcgacgggcgtgtgaacgtttcgtagtcatagcgctagcacgtacctagccacatggtcgtgaggag ctttaccatgcgtctagaaggtggatgcgggacacgccttcctggcctttggctcccgagacgcgtgttc taaccacaaaccttgagacgcgtgttccaaccacgcaccctgacacgcgtgttccaaccacgcaccctga gacgcgtgttctaaccacgcaccctgagacgcgtgttctaaccacgcaccctgagacgcgtgttctgccg cacaatgtgcacctgtaggaagctgtagtcactgctgattctcactgttctcggcaagggccgacgaccg gagtacagtttttgtgggcagagccgttgtgcagctttccgttcttctcggttgtgtcacatgtgtcatt gtcgtgtaaacacacggttgtatgtcggtttcgctgcaccacttcattatttcttctggttttttgacga gtatgcatctagacaaaatggacaagaagtacagcatcggcctggacatcggcaccaactctgtgggctg ggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcac agcatcaagaagaacctgatcggcgccctgctgttcgacagcggagaaacagccgaggccacccggctga agagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaa cgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggataag aagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtaccccacca tctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctatctggccct ggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtg gacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcg gcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgccca gctgcccggcgagaagaagaatggcctgttcggcaacctgattgccctgagcctgggcctgacccccaac ttcaagagcaacttcgacctggccgaggatgccaaactgcagctgagcaaggacacctacgacgacgacc tggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctggccgccaagaacctgtccga cgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatg atcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcctg agaagtacaaagagattttcttcgaccagagcaagaacggctacgccggctacatcgatggcggagccag ccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtg aagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatcc acctgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccggga aaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcaga ttcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagg gcgccagcgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgagaaggtgct gcccaagcacagcctgctgtacgagtacttcaccgtgtacaacgagctgaccaaagtgaaatacgtgacc gagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaagccatcgtggacctgctgttcaaga ccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttcgactccgt ggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaattatc aaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacac tgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgat gaagcagctgaagcggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgg gacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacagaaacttcatgc agctgatccacgacgacagcctgacctttaaagaggacatccagaaagcccaggtgtccggccagggcga tagcctgcacgagcacattgccaatctggccggcagccccgccattaagaagggcatcctgcagacagtg aaggtggtggacgagctcgtgaaagtgatgggccggcacaagcccgagaacatcgtgatcgaaatggcca gagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcat caaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagctgcagaacgagaagctg tacctgtactacctgcagaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccg actacgatgtggaccatatcgtgcctcagagctttctgaaggacgactccatcgataacaaagtgctgac tcggagcgacaagaaccggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaac tactggcgccagctgctgaatgccaagctgattacccagaggaagttcgacaatctgaccaaggccgaga gaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggtggaaacccggcagatcac aaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgagaacgacaaactgatccgg gaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagttttacaaag tgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaaccgccctgat caaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatg atcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaact ttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcga aacaggcgagatcgtgtgggataagggccgggactttgccaccgtgcggaaagtgctgtctatgccccaa gtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagtctatcctgcccaagagga acagcgacaagctgatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgacagccccac cgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaaa gagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagcca agggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaa cggccggaagagaatgctggcctctgccggcgaactgcagaagggaaacgaactggccctgccctccaaa tatgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcaga aacagctgtttgtggaacagcacaaacactacctggacgagatcatcgagcagatcagcgagttctccaa gagagtgatcctggccgacgctaatctggacaaggtgctgagcgcctacaacaagcacagagacaagcct atcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttca agtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggacgccaccctgat ccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggcgacgcctatccc tatgacgtgcccgattatgccagcctgggcagcggctcccccaagaaaaaacgcaaggtggaagatccta agaaaaagcggaaagtggacggcattggtagtgggagcaacggcagcagcggatccgtgagcaagggcga ggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagc gtgcgcggcgagggcgagggcgatgccaccaacggcaagctgaccctgaagttcatctgcaccaccggca agctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccc cgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatc tccttcaaggacgacggcacctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaacc gcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactt caacagccacaacgtctatatcacggccgacaagcagaagaacggcatcaaggcgaacttcaagatccgc cacaacgtcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggcc ccgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcg cgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag tagttaattaatcaccgttgtgctcacttctcaaatcgacaaaggaaacacacttcgtgcagcatgtgcc ccattataaagaaactgagttgttccgttgtggcttgcaggtgtcacatccacaaaaaccggccgactct aaataggagtgtttcgcagcaagcagcgaaagtttatgactgggtccgaatctctgaacggatgtgtggc ggacctggctgatgttgatcgccgtcgacacacgcgccacatgggtcaatacacaagacagctatcagtt gttttagtcgaaccggttaacacaattcttgcccccccgagggggatccactagttctagagcggccgcc accgcggtggagct

Addgene-Verified Partial Sequences (5)

Addgene has verified portions of this plasmid. Results are shown below. Primers used for Sanger sequencing are indicated in the sequence header.

Analyze Sequence
Sequence > dCas9-R2 5'-GGGTTTCCACCAGCTGTCTC-3' CAGTTCGCTCAGGCCGCCTCTCTCGGCCTTGGTCAGATTGTCGAACTTCCTCTGGGTAATCAGCTTGGCA TTCAGCAGCTGGCGCCAGTAGTTCTTCATCTTCTTCACGACCTCTTCGGAGGGCACGTTGTCGCTCTTGC CCCGGTTCTTGTCGCTCCGAGTCAGCACTTTGTTATCGATGGAGTCGTCCTTCAGAAAGCTCTGAGGCAC GATATGGTCCACATCGTAGTCGGACAGCCGGTTGATGTCCAGTTCCTGGTCCACGTACATATCCCGCCCA TTCTGCAGGTAGTACAGGTACAGCTTCTCGTTCTGCAGCTGGGTGTTTTCCACGGGGTGTTCTTTCAGGA TCTGGCTGCCCAGCTCTTTGATGCCCTCTTCGATCCGCTTCATTCTCTCGCGGCTGTTCTTCTGTCCCTT CTGGGTGGTCTGGTTCTCTCTGGCCATTTCGATCACGATGTTCTCGGGCTTGTGCCGGCCCATCACTTTC ACGAGCTCGTCCACCACCTTCACTGTCTGCAGGATGCCCTTCTTAATGGCGGGGCTGCCGGCCAGATTGG CAATGTGCTCGTGCAGGCTATCGCCCTGGCCGGACACCTGGGCTTTCTGGATGTCCTCTTTAAAGGTCAG GCTGTCGTCGTGGATCAGCTGCATGAAGTTTCTGTTGGCGAAGCCGTCGGACTTCAGGAAATCCAGGATT GTCTTGCCGGACTGCTTGTCCCGGATGCCGTTGATCAGCTTCCGGCTCAGCCTGCCCCAGCCGGTGTATC TCCGCCGCTTCAGCTGCTTCATCACTTTGTCGTCGAACAGGTGGGCATAGGTTTTCAGCCGTTCCTCGAT CATCTCTCTGTCCTCAAACAGTGTCAGGGTCAGCACGATATCTTCCAGAATGTCCTCGTTTTCCTCATTG TCCAGGAAGTCCTTGTCCTTGATAATTTTCAGCAGATCGTGGTATGTGCCcAGGGAGGCGTTGAACCGAT CTTCCACGCCGGAGATTTCCACGGAGTCGAAGCACTCGATTTTCTTGAAGTAGTCCTCTTTCAGCTGCTT CACGGTCACTTTCCGGTTGG
Analyze Sequence
Sequence > hSpCas9-R1 5'-CGCTCGTGCTTCTTATCCTC-3' CAGTCTGTGGAAGAAGCTGTCGTCCACCTTGGCCATCTCGTTGCTGAAGATCTCTTGCAGATAGCAGATC CGGTTCTTCCGTCTGGTGTATCTTCTTCTGGCGGTTCTCTTCAGCCGGGTGGCCTCGGCTGTTTCTCCGC TGTCGAACAGCAGGGCGCCGATCAGGTTCTTCTTGATGCTGTGCCGGTCGGTGTTGCCCAGCACCTTGAA TTTCTTGCTGGGCACCTTGTACTCGTCGGTGATCACGGCCCAGCCCACAGAGTTGGTGCCGATGTCCAGG CCGATGCTGTACTTCTTGTCCATTTTGTCTAGATGCATACTCGTCAAAAAACCAGAAGAAATAATGAAGT GGTGCAGCGAAACCGACATACAACCGTGTGTTTACACGACAATGACACATGTGACACAACCGAGAAGAAC GGAAAGCTGCACAACGGCTCTGCCCACAAAAACTGTACTCCGGTCGTCGGCCCTTGCCGAGAACAGTGAG AATCAGCAGTGACTACAGCTTCCTACAGGTGCACATTGTGCGGCAGAACACGCGTCTCAGGGTGCGTGGT TAGAACACGCGTCTCAGGGTGCGTGGTTAGAACACGCGTCTCAGGGTGCGTGGTTGGAACACGCGTGTCA GGGTGCGTGGTTGGAACACGCGTCTCAAGGTTTGTGGTTAGAACACGCGTCTCGGGAGCCAAAGGCCAGG AAGGCGTGTCCCGCATCCACCTTCTAGACGCATGGTAAAGCTCCTCACGACCATGTGGCTAGGTACGTGC TAGCGCTATGACTACGAAACGTTCACACGCCCGTCGCACATTGTTGCCACTGTCAAGAACCTGCCCTGGT GTTACGTTGCGGCACTGTGAGCACTGATAGGGCAACGAGATCGGCCCCTAATTCAGCGAGGCCCCAGTAC CGTGTCTTGCAGTGCATATTACCTAGACACCGTCAGTTTCAAATGCCTCGTGTGGGAAGTTTCGTTCGTT GTCTCAGACTTTGCAGTAGATAGTCATCAGCAAGGTGCCCACCATTCGACCTGTCACTGCAGTCTTATTA GTCGCAACGACCAGTCTTTGAAGCTGC
Analyze Sequence
Sequence > hSpCas9-R1 5'-CGCTCGTGCTTCTTATCCTC-3' CAGTCTGTGGAAGAAGCTGTCGTCCACCTTGGCCATCTCGTTGCTGAAGATCTCTTGCAGATAGCAGATC CGGTTCTTCCGTCTGGTGTATCTTCTTCTGGCGGTTCTCTTCAGCCGGGTGGCCTCGGCTGTTTCTCCGC TGTCGAACAGCAGGGCGCCGATCAGGTTCTTCTTGATGCTGTGCCGGTCGGTGTTGCCCAGCACCTTGAA TTTCTTGCTGGGCACCTTGTACTCGTCGGTGATCACGGCCCAGCCCACAGAGTTGGTGCCGATGTCCAGG CCGATGCTGTACTTCTTGTCCATTTTGTCTAGATGCATACTCGTCAAAAAACCAGAAGAAATAATGAAGT GGTGCAGCGAAACCGACATACAACCGTGTGTTTACACGACAATGACACATGTGACACAACCGAGAAGAAC GGAAAGCTGCACAACGGCTCTGCCCACAAAAACTGTACTCCGGTCGTCGGCCCTTGCCGAGAACAGTGAG AATCAGCAGTGACTACAGCTTCCTACAGGTGCACATTGTGCGGCAGAACACGCGTCTCAGGGTGCGTGGT TAGAACACGCGTCTCAGGGTGCGTGGTTAGAACACGCGTCTCAGGGTGCGTGGTTGGAACACGCGTGTCA GGGTGCGTGGTTGGAACACGCGTCTCAAGGTTTGTGGTTAGAACACGCGTCTCGGGAGCCAAAGGCCAGG AAGGCGTGTCCCGCATCCACCTTCTAGACGCATGGTAAAGCTCCTCACGACCATGTGGCTAGGTACGTGC TAGCGCTATGACTACGAAACGTTCACACGCCCGTCGCACATTGTTGCCACTGTCAAGAACCTGCCCTGGT GTTACGTTGCGGCACTGTGAGCACTGATAGGGCAACGAGATCGGCCCCTAATTCAGCGAGGCCCCAGTAC CGTGTCTTGCAGTGCATATTACCTAGACACCGTCAGTTTCAAATGCCTCGTGTGGGAAGTTTCGTTCGTT GTCTCAGACTTTGCAGTAGATAGTCATCAGCAAGGTGCCCACCATTCGACCTGTCACTGCAGTCTTATTA GTCGCAACGACCAGTCTTTGAAGCTGC
Analyze Sequence
Sequence > M13-Rev 5'-CAGGAAACAGCTATGACC-3' ACCCTCACTAAAGGGAACAAAAGCTGGAGCTCAAAAAAGCACCGACTCGGTGCCACTTTTCAAGTTGATA ACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACCACATCGCTGTCAGACCAATAACTTGA CATCCCCATTTACCAGAAGGCAAACACCCCCTTCGGGGACGAGGTGACCCTGCGCGACAGAAAGCCCCTT CGAAGAGCGCACAGGGAGGAAGCAGGCCTCTGCAGGTCGCCATTTGAAAATCTGACAGAACTGATGGAAA TATGATTCTTGTCAGAGAAGACATTCGAGAGTTCGAAGGTTTCCCCCTTGGCTCTACATATCCCAGTGTC TCGCGTTCTGCAGGAGGCGCGTCAGGCCTAGGATGCAATATTGGCGCCCAATTCACAGTGCAGCGGCGCA GCCGTCGCAACACTTCGCAGCGTGAAAGACTTATCGCTCTCCGCCTCTCGATGGTTCGGGATGTTCGCTT CGAGTGTATGCTGCGCAAGCTTTCGCGCTTGGGTACGTTAATTTGAAATCTTTCGCGTTTCCCGTGTGTC CTCAGTGTCTCCAACATGTGGCCGCGGTGCAATCGTCCCCACGACGATTTCAACTCGCGCACTTTGTCTC GTCGCACCCTCCCGGAAATACAGCGTGCTTCTGCTTACTTGAGCTCCACCGCGGTGGCGGCCGCTCTAGA ACTAGTGGATCCCCCTCGGGGGGGCAAGAATTGTGTTAACCGGTTCGACTAAAACAACTGATAGCTGTCT TGTGTATTGACCCATGTGGCGCGTGTGTCGACGGCGATCAACATCAGCCAGGTCCGCCACACATCCGTTC AGAGATTCGGACCCAGTCATAAACTTTCGCTGCTtGCTGCGAAACACTCCTATTTAGAGTCGGCCGGTTT TTGTGGATGTGACACCTGCAAGCC
Analyze Sequence
Sequence > M13-F20 5'-TGTAAAACGACGGCCAGT-3' GGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTTTACATCCGTTGCCTTTTC CACGGTCCGTGATTTCATGTGCGTGCAGCTTCAAAGACTGGTCGTTGCGACTAATAAGACTGCAGTGACA GGTCGAATGGTGGGCACCTTGCTGATGACTATCTACTGCAAAGTCTGAGACAACGAACGAAACTTCCCAC ACGAGGCATTTGAAACTGACGGTGTCTAGGTAATATGCACTGCAAGACACGGTACTGGGGCCTCGCTGAA TTAGGGGCCGATCTCGTTGCCCTATCAGTGCTCACAGTGCCGCAACGTAACACCAGGGCAGGTTCTTGAC AGTGGCAACAATGTGCGACGGGCGTGTGAACGTTTCGTAGTCATAGCGCTAGCACGTACCTAGCCACATG GTCGTGAGGAGCTTTACCATGCGTCTAGAAGGTGGATGCGGGACACGCCTTCCTGGCCTTTGGCTCCCGA GACGCGTGTTCTAACCACAAACCTTGAGACGCGTGTTCCAACCACGCACCCTGACACGCGTGTTCCAACC ACGCACCCTGAGACGCGTGTTCTAACCACGCACCCTGAGACGCGTGTTCTAACCACGCACCCTGAGACGC GTGTTCTGCCGCACAATGTGCACCTGTAGGAAGCTGTAGTCACTGCTGATTCTCACTGTTCTCGGCAAGG GCCGACGACCGGAGTACAGTTTTTGTGGGCAGAGCCGTTGTGCAGCTTTCCGTTCTTCTCGGTTGTGTCA CATGTGTCATTGTCGTGTAAACACACGGTTGTATGTCGGTTTCGCTGCACCACTTCATTATTTCTTCTGG TTTTTTGACGAGTATGCATCTAGACAAAATGGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAAC TCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACA CCGACCGGCACAGCATCAAGAAGAACCTGATCGGCGCCCTGCTGTTCGACAGCGGAGAAACAGCCGAGGC C

pSAG1::CAS9-U6::sg290860-6 Sequences (6)

Depositor Full Sequences (1)

Addgene-Verified Partial Sequences (5)

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