Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

4QPN
(Plasmid #61963)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61963 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    Structural Genomics Consortium
  • Vector type
    Baculovirus Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Gentamicin (bacmid resistance in DH10Bac E. coli)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    METTL21B
  • Alt name
    PDB:4QPN
  • Species
    H. sapiens (human); Plasmodium falciparum
  • Insert Size (bp)
    678
  • Mutation
    See comments
  • Entrez Gene
    METTL21B (a.k.a. FAM119B)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • MHHHHHHSSGRENLYFQG (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer Polyhedrin Forward (AAATGATAACCATCTCGC)
  • 3′ sequencing primer SV40 poly A Reverse
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4QPN was a gift from Cheryl Arrowsmith (Addgene plasmid # 61963 ; http://n2t.net/addgene:61963 ; RRID:Addgene_61963)