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pBSII-flotillin1a
(Plasmid #62800)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62800 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pBSII-SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 4700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    flotillin1a
  • Alt name
    flot1-a
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1700
  • GenBank ID
    AF545659
  • Entrez Gene
    flot1-a (a.k.a. flot1a, flotillin-1, flotillin1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13pUC-Rev; T3
  • 3′ sequencing primer M13pUC-Fwd; T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSII-flotillin1a was a gift from Sally Moody (Addgene plasmid # 62800)

  • For your References section:

    Xenopus flotillin1, a novel gene highly expressed in the dorsal nervous system. Pandur PD, Dirksen ML, Moore KB, Moody SA. Dev Dyn. 2004 Dec;231(4):881-7. 10.1002/dvdy.20191 PubMed 15517583
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