pIRESpuro2_furin_cDNA
(Plasmid #63685)

• Purpose: human furin cDNA (NM_002569.2) cloned into pIRESpuro2, between the AflII and BstB1 restriction sites after PCR amplification using primers 5’-GAGAGACTTAAGATGGAGCTGAGGCCCTGG (Forward) and 5’-GACCGATTCG
• Depositing Lab: Richard Roden
• Depositing Organization: Johns Hopkins University and School of Medicine
  Located in United States of America
• Publication: Wang et al Virology. 2014 Jan 20;449:304-16. doi: 10.1016/j.virol.2013.10.038. Epub 2013 Dec 20.

Backbone

• Vector backbone: pIRESpuro2
• Backbone manufacturer: CloneTech
• Backbone size w/o insert (bp): 5192
• Total vector size (bp): 7554
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FURIN
• Alt name: Homo sapiens furin (paired basic amino acid cleaving enzyme) (FURIN), mRNA
• Species: H. sapiens (human)
• Insert Size (bp): 2397
• GenBank ID: NM_002569.2
• Entrez Gene: FURIN (a.k.a. FUR, PACE, PCSK3, SPC1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: aflII (unknown if destroyed)
• 3′ cloning site: BstBI (unknown if destroyed)
• 5′ sequencing primer: GAGAGACTTAAGATGGAGCTGAGGCCCTGG
• 3′ sequencing primer: GACCGATTCGAATCATCAGAGGGCGCTCTGGTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA